The Effect of One-Week Spinach and NBS Superfood Supplementation on Interleukin-6, Superoxide Dismutase, and Malondialdehyde Levels after Repeated Bouts of Wingate Test in Trained Men

Background: Consuming nutritional supplements for improving athletic performance has always been considered an ergogenic aid. However, there is limited information on the efficacy of this strategy for enhancing antioxidant capacity in response to strenuous exercise with repeated nature. Therefore, this study investigated the effect of one week of spinach and NBS superfood supplementation on superoxide dismutase, malondialdehyde, and interleukin-6 in response to repeated bouts of Wingate test. Methods: Fifteen trained men voluntarily participated in this within-subject and counter-balanced study. Participants were exposed to three different conditions including 1) seven days of raw spinach supplementation (daily dose of 70 g); 2) seven days of NBS superfood supplementation (daily dose of 10 g); and 3) the control condition. One day after the supplementation period, participants came to the lab and performed 3 bouts of 30-second Wingate test with 4-minute rest intervals. Before the supplementation and 24 hours after performing the Wingate, test blood samples were taken in each condition to measure serum levels of superoxide dismutase, malondialdehyde, and interleukin-6. One week of wash-out was applied between the conditions. Two-way repeated measures ANOVA were used for statistical analysis. Results: The results showed that at post-test measurement the serum level of interleukin-6 was significantly lower under the spinach and superfood conditions (p=0.001, p=0.003, respectively) compared to the control. The serum level of superoxide dismutase under the spinach (p=0.035) and superfood (p=0.01) conditions was significantly higher compared to the control at post-test measurement. Also, at post-test measurement, the serum level of malondialdehyde was significantly lower under the spinach (p=0.001) and superfood (p=0.017) conditions compared to the control. Conclusion: According to the results of the present study, it seems that seven days’ supplementation of either raw spinach or NBS superfood could positively affect the inflammatory responses to repeated anaerobic all-out activities. Therefore, these supplementation strategies might be considered ergogenic aids and used by athletes before participating in repeated strenuous activities.