Isolation of Bacillus zhangzhouensis OBB from local Polyphylla fullo (L.) larvae and PCR-based detection of cry1 gene

The most commonly used bacteria in biopesticide production is Bacillus thuringiensis due to cry proteins. Since continuous and repeated use of biopesticides may cause resistance development in pests, it is necessary to investigate isolates containing new cry genes. Bacillus zhangzhouensis OBB was isolated from Polyphylla fullo larvae. The presence of the cry gene was detected in this isolate. The conclusion that the strain carries the cry gene was made by PCR and SEM analysis. In addition, the central location of the Bacillus zhangzhouensis OBB spore structure, SDS-PAGE analysis and DNA sequence analysis showed phylogenetic differences between B. thuringiensis. B. zhangzhouensis showed bands between ̴ 250 kDa and ̴ 80 kDa. As a result, Bacillus zhangzhouensis OBB can be used as an alternative biopesticide in case of insect resistance to B. thuringiensis biopesticides. Indicating that this isolate has the potential to be effective against Coleoptera insect pests.