Analysis of Rhizosphere microbial community structure and production performance of different maize varieties

Aims: To clarify the rhizosphere microbial community structure of different maize varieties and its relationship with yield traits. Methods: The rhizospheremicrobial community structure of seven maize varieties was analysed at seedling and staminate stages using high-throughput sequencing of 16SrDNA and ITS rDNA amplicons to determine maize yield traits and their correlation studies. Results: Compared with the seedling stage, the relative abundance of Aspergillus phylum and Anaplasma phylum decreased more, Acidobacter phylum bacteria increased more, Sphingomonas spp. and Marseille spp. decreased more, and Acidobacterium RB41 increased more in the same varieties at the male pumping stage. At the male-drawing stage, the relative abundance of Acidobacter RB41 was the highest in all varieties except variety 8; the relative abundance of Sphingomonas spp . was the lowest in variety 2 and the highest in variety 8. The relative abundance of Ascomycetes phylum was the highest in all seven varieties, with the highest in variety 2 at the male-drawing stage, and the male-drawing stage was higher than that at the seedling stage in all varieties except variety 14. s_Pedobacter panaciterrae was positively correlated with yield, s_Spartobacteria bacterium was positively correlated with number of ears, s_Microdochium bolleyi was negatively correlated with water content, and s_ Podospora multipilosa was positively correlated with number of ears. Conclusion: s_Pedobacter panaciterrae, s_Spartobacteria bacterium have positive role in yield traits, which provides theoretical basis for further development of microbial fertiliser for maize yield increase.