Integrated Biophysical and Biochemical Analyses for Profiling Lipids and Carotenoids in Rhodotorula mucilaginosa

The oleaginous yeast species Rhodotorula mucilaginosa is distinguished by its genetic, ecological, physiological, and morphological variety. The biotechnological potential of R. mucilaginosa , which was isolated from petroleum oil refinery resources in Guwahati, Assam, India, specifically for the potential producing of lipids, carotenoids, and β-carotene were examined. In this study to describe carotenoid pigments in R. mucilaginosa , determine targets for metabolic products, and comprehend the precise lipid composition profile. R. mucilaginosa alive cells were analysed using a variety of biochemical and biophysical techniques, such as gas chromatography for lipid and thin-layer chromatography for neutral lipid composition profiling, Raman spectroscopy for lipid and carotenoid analysis, Field Effect Scanning Electron Microscopy (FESEM) for lipid visualization, and fluorescence spectroscopy for lipid detection using the Nile red assay. The results of these tests offer a thorough understanding of R. mucilaginosa's capacity to produce carotenoid and lipids. This precise identification of oleaginous yeast R. mucilaginosa needed for the commercial production of carotenoids, high-value biofuel, and value-added merchandises. Raman spectroscopy, fluorescence spectroscopy, FESEM, TLC, and LC-MS provided valuable insights into the lipid and carotenoid production capabilities of R. mucilaginosa . Moreover, any of these analytical techniques can be used to confirm the presence of lipids and carotenoids in yeast species. These comprehensive results help reduce the time required for selecting new oleaginous yeast species.