Brevibacterium litoralis sp. nov., a cellulose-degrading strain isolated from marine surface sediment

A Gram stain-positive, non-spore-forming, non-motile, short-rod actinomyces strain GXQ1321 ^T was isolated from maritime surface sediments in Beihai(11°46′21.11″N, 109°62′56.25″E), Guangxi Zhuang Autonomous Region, and a number of categorization studies were performed. Following a period of 72 hours of incubation at a temperature of 30°C within an actinomycetes culture medium, the colony was yellow, circular, smooth, central bulge, convex, opaque, with a 1.8-3.0 mm diameter. Strain GXQ1321 ^T has the ability to produce amylase and cellulase. Chemotaxonomic studies revealed that the major menaquinone in strain GXQ1321 ^T is MK-8. The most prevalent cellular fatty acids were anteiso -C _19:0 (27.28%), anteiso -C _15:0 (18.97%), anteiso -C _17:0 (15.95%), and iso -C _16:0 (12.21%). The whole-cell sugars of the strain GXQ1321 ^T identified were rhamnose, xylose and glucose. Strain GXQ1321 ^T exhibited the presence of meso-diaminopimelic acid (m-DAP) as a distinctive cell-wall diamino acid, and the polar lipids were identified as diphosphatidylglycerol (DPG), three phosphoglycolipid (PGL), phosphatidylglycerol (PG) and two unknown glycolipid (UG). This strain had 69.6% DNA G + C content. Strain GXQ1321 ^T is classified as Brevibacterium based on its 16S rRNA gene sequence. It is closely related to Brevibacterium samyangense SST-8 ^T (96.77%) and Brevibacterium rongguiense 5221 ^T (96.32%). The results showed that the average nucleotide identity (ANI) values of GXQ1321 ^T and the above two strain tyoes were 73.91–77.14%, and the digital DNA-DNA hybridisation (dDDH) values were 15.3–21.1%. Based on the phylogenetic, chemotaxonomi and physiologicalc data, strain GXQ1321 ^T was considered to be a new species of the genus Brevibacterium , named Brevibacterium litoralis sp. nov, with the type strain GXQ1321 ^T (= MCCC 1K08964 ^T  = KCTC 59167 ^T ).