KRT23 acts as an oncogene in hepatocellular carcinoma by regulating PI3K/AKT/GSK3β pathway via P21

Hepatocellular carcinoma (HCC) is a leading cancer worldwide. Keratin23 maybe a potential biomarker for HCC development; however, regulatory mechanisms remain unclear. In this research we explored the expression and effect of KRT23 in HCC. GEPIA, Kaplan-Merier survival analysis IHC, qRT-PCR and western blot were applied to further detect the expression of KRT23, as well as prognosis. Functionally, Relative cell biology experiments were applied. Moreover, xenograft tumors were conducted in vivo . Mechanically, Immunofluorescence, western blot and Co-immunoprecipitation were operated. As for results, we discovered the high expression of KRT23 in HCC. Functionally, KRT23 knockdown reduced cell proliferation and metastasis. KRT23 knockdown inhibited EMT and PI3K/AKT/GSK3β pathway. In summary, KRT23 accelerated HCC proliferation and metastasis by regulating PI3K/AKT/GSK3β pathway via P21.