Microbial Community Diversity and Succession of Casing Soil during Oudemansiella raphanipes Cultivation

In this study, high-throughput sequencing(HTS) was used to demonstrate the microbial community diversity and succession of casing soil during Oudemansiella raphanipes cultivation. The abundance and diversity of bacteria and fungi were the highest in the original soil of vegetable soil, and decreased in casing soil, but increased at the end of the mushroom formation. /As the mushroom formation progressed, bacterial aspects, some microorganisms that are often found in heavy metal contaminated soils such as Massilia, Sphingomonas , and Cupriavidus increased in abundance. In terms of fungi, the dominant fungi in the vegetable soil and at the end of the mushroom formation stage was Thermoascus, and the abundance of some plant and animal pathogens, such as Candida, Aspergillus, Trichoderma, and Chaetomium, increased in the first and second mushroom formation stages.This study revealed microbial community succession of casing soil during Oudemansiella raphanipes cultivation, providing theoretical support to improve the yield and quality of Oudemansiella raphanipes by adjusting the casing soil, and also to find solutions for disease control.