Temperature-induced changes in the hematological and biochemical parameters of Nile tilapia anesthetized with Ocimum basilicum

  1. Paola Capistrano Santos
  2. Emilly Monteiro Lopes
  3. Arlene Sobrinho Ventura
  4. Claudia Andrea Lima Cardoso
  5. Alexandre Vaz Silva
  6. Domickson Silva Costa
  7. Marília Tedesco
  8. Gabriela Tomas Jerônimo
  9. Maurício Laterça Martins
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Abstract

The study aimed to evaluate the effect of temperatures of 23 and 27 ºC on the hematology of Nile tilapia juveniles ( Oreochromis niloticus ) subjected to anesthesia using the essential oil of basil ( Ocimum basilicum ) at a concentration of 250 mg L -1 . A total of 112 fish were divided into 4 treatments and distributed as follows: fish anesthetized with 250 mg L -1 at a temperature of 23ºC (n=42); fish anesthetized with 250 mg L -1 at a temperature of 27ºC (n=42); and for each temperature, a control group fish exposed to diluent solution (95% ethanol) (n=14, seven per temperature); a control group fish (water) (n=14, seven per temperature). They were exposed to 10 min for both temperatures. Blood collection was performed after the anesthetic induction at each temperature to determine the hematocrit, hemoglobin concentration (Hb), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), relative values of leukocytes and absolute values of leukocytes and thrombocytes, in addition to evaluating plasma chloride and total calcium ions. The analysis of chemical composition by gas chromatography coupled with mass spectrometry (GC-MS) demonstrated methyl chavicol (70.04%) and linalool (24.59%) as the major components of basil essential oil. Fish anesthetized at a temperature of 23 ºC showed lower neutrophil counts (p<0.05) compared to the other tested groups. For the temperature of 27 ºC, values of MCV, MCHC, and RBC values decreased (p<0.05) following exposure to basil essential oil. The calcium ion showed a statistical difference (p<0.05) between the treatments about the water control group. It is concluded that basil essential oil presented a positive anesthetic effect. The use of O. basilicum essential oil as an anesthetic in Nile tilapia was the best at 23ºC, causing less hematological and biochemical stress.

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  1. Version published to 10.21203/rs.3.rs-4713885/v1 on Research Square