Reduction of Silver Oxide Nanoparticles Using Anethum Graveolens Leaves Extract and Assessment of Their Antibacterial and Antibiofilm Properties

This research aims to develop a new method for preparing silver oxide nanoparticles using Anethum graveolens (dill) leaf extract as a natural reducing agent, using cold plasma technology. This study was designed to evaluate the antibacterial and anti-biofilm properties of the prepared nanoparticles. Initially, dill leaf extract was prepared and used as a reducing agent and stabilizer for silver oxide nanoparticles. Cold plasma technology was used to enhance the synthesis process and provide ideal conditions for the formation of nanoparticles. The results showed that the prepared silver oxide nanoparticles possess strong antibacterial properties, as they showed remarkable activity against a variety of pathogenic bacteria such as " Klebsiella pneumoniae, Escherichia coli , and Staphylococcus aureus ". Nanoparticles also proved effective in preventing the formation of bacterial biofilms, as the highest rate of inhibition was for the Gram-positive bacteria Staphylococcus aureus. The study provides evidence of the effectiveness of combining plant extracts and cold plasma technology in producing nanoparticles with improved properties, which could push towards the use of these materials. In developing innovative and sustainable solutions in various scientific and applied fields.