Deciphering PCSK9 and LDLR Gene Expression in Coronary Artery Disease Patients: Implications for Blood Lipid Regulation
Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
The main purpose of this study to calculate the frequency, risk factors, sedentary lifestyle, prevalence and expression of PCSK9 and LDLR genes showed a substantial role in cholesterol homeostasis involving in prognosis of coronary artery disease patients in Pakistan. Therefore, there is an urgent need to assess the frequency and risk factors involved and expression and regulation of a PCSK9 and LDLR in patients. A demographic survey involving CAD patients from different cardiac Hospitals in Pakistan was conducted and 100 CAD patients and 100 controls were included for the investigation of the expression level of PCSK9 and LDLR gene by using RT-PCR. CAD patients (males = 803, Females = 307) that participated in this study consisted of smokers (55%) and non-smokers (45%), the majority had a family history (62% positive, 38% negative). The participants also had diverse weight profiles (underweight 14%, active or normal 21%, overweight 27%, and obese 34%); while the majority claimed that they suffered stress (stress 85%, no stress 15%). Strong negative correlations (p < 0.01) were observed between CAD & gender, Diet, age of diagnosis, BMI, marital status, stress, different risk factors and family history. A negative correlation (p < 0.05) was seen between CAD and Sleep and with exercise also. The results indicated that the PCSK9 and LDLR expression level were considerably higher P was less than 0.05 in the patient group as compared to the healthy group. The AUC value of expression of PCSK9 (P = 6.58337E-4) and LDLR (P = 0.00111) were significant. The cox model result showed that high expression of LDLR and PCSK9 have significant effect, both the differences and overall survival rate were (P > 0.05) statistically significant. The findings of this study will help improve the management and treatment of CAD worldwide, the expression of PCSK9 and LDLR in CAD’s patient’s with specific implications in under developed countries.
