Dissecting Triple Emission from a Fluorescent Protein
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The present study delves into the intricate photo-physics of the red fluorescent protein mBeRFP, exhibiting extraordinarily large Stokes shift of ~180 nm. It is observed that at room-temperature mBeRFP has two emission bands in green and red, while at cryogenic-temperature an additional blue-emission is reported. A multifaceted approach, integrating steady-state spectroscopy, electronic structure calculations and femtosecond transient absorption spectroscopy, were employed to elucidate the remarkable spectral properties of this protein
