The efficacy of a novel porcine-derived collagen membrane on guided bone regeneration: A comparative study in canine model

Background: This study aimed to evaluate the performance of the novel Striate+™ collagen membrane in a canine model of guided bone and tissue regeneration (GBR) with dental implant placement. Methods: Eighteen mature beagle dogs were used in this split-mouth design study. After having their premolar extracted, immediate implants with GBR techniques were carried out on all study subjects. The study treatments were: control group (implant + no membrane); BG-group (implant + Bio-Gide® membrane); and SG-group (implant + Striate+™ membrane). Six dogs were sacrificed at 4-, 8- and, 12-weeks post-treatment for radiographic (μCT) assessment, histological examination and histomorphometric analysis. Results: μCT assessment showed that all groups exhibited increased bone formation from 4-weeks to 12-weeks post-treatment. There was no statistically significant difference in mean BV/TV between all 3 groups at weeks 4 and 8. But at week 12, BV/TV was significantly higher in SG and BG-groups compared to control group. Assessment of bone microarchitectural parameters showed that animals in SG-group exhibited significantly higher Tb.N, O.Wi and lower Tb.Sp, suggesting more favorable mature bone structure. A significant increase in the number of osteoblasts on bone surface was also seen in SG-group. Histological assessment showed that SG-group displays early signs of bone-to-implant contact at 8 weeks. While control sites showed early ingrowth of epithelium and connective tissue into the defects, infiltration of inflammatory cells, incomplete bone formation and limited bone to implant contact; significant bone infill, mature bone with good implant contact and limited soft tissue invasion were observed in SG- and BG- groups. Conclusion: This study demonstrated superiority of Striate+™ collagen membrane in GBR and prevention of unwanted epithelial infiltration in a canine model.