Neuro-intestinal Acetylcholine Signalling Regulates the Mitochondrial Stress Response

Neurons coordinate inter-tissue protein homeostasis to systemically manage cytotoxic stress. In response to neuronal mitochondrial stress, specific neuronal signals coordinate the systemic mitochondrial unfolded protein response (UPR ^mt ) to promote organismal survival. Yet, whether chemical neurotransmitters are sufficient to control the UPR ^mt in physiological conditions is not well understood. Here, we show that gamma-aminobutyric acid (GABA) inhibits, and acetylcholine (ACh) promotes the UPR ^mt in the Caenorhabditis elegans intestine. GABA controls the UPR ^mt by regulating extra-synaptic ACh release through metabotropic GABA _B receptors GBB-1/2. We find that elevated ACh levels in animals that are GABA-deficient or lack ACh-degradative enzymes induce the UPR ^mt through ACR-11, an intestinal nicotinic a7 receptor. This neuro-intestinal circuit is critical for non-autonomously regulating organismal survival of oxidative stress. These findings establish chemical neurotransmission as a crucial regulatory layer for nervous system control of systemic protein homeostasis and stress responses.