Sulfamethoxazole Pharmaceutical Dosage Form Quantitative Estimation Using UV-Visible Spectrophotometric Method

Sulfamethoxazole, a widely used antibiotic, is often formulated as a combination product with trimethoprim to treat bacterial infections. Accurate and reliable quantitation of sulfamethoxazole is essential for quality control and assurance in pharmaceutical industries. In this study, a simple and cost-effective UV-Visible spectrophotometric method was developed for the quantitative estimation of sulfamethoxazole in pharmaceutical dosage forms.The method involves measuring the absorbance of sulfamethoxazole at 264 nm, which is a characteristic wavelength of the compound. The calibration curve was constructed by plotting the absorbance values against the concentration of sulfamethoxazole in the range of 2-12 μg/mL. The results showed a good linear correlation (r² = 0.999) with a limit of detection (LOD) and quantification (LOQ) of 0.5 μg/mL and 1.5 μg/mL, respectively.The method was validated by analyzing commercial pharmaceutical products containing sulfamethoxazole and trimethoprim. The results showed good accuracy and precision, with mean recovery percentages ranging from 98% to 102%. The method was also found to be selective, with no interference from excipients or other components commonly present in pharmaceutical formulations.In conclusion, the proposed UV-Visible spectrophotometric method is a reliable and efficient tool for the quantitative estimation of sulfamethoxazole in pharmaceutical dosage forms. The method can be easily adapted for routine quality control analysis and can help ensure the quality and consistency of sulfamethoxazole-based products.