The Effectiveness of Extract from Ambon Banana Stem Musa cavendishii var. Dwarf paxton for Prevention of Vibrio parahaemolyticus Bacterial Infection in Vannamei Shrimp Litopenaeus vannamei

This study aims to evaluate the effect of administering Ambon banana stem extract through feed in enhancing the immune response, growth, and resistance of vannamei shrimp to diseases caused by Vibrio parahaemolyticus bacteria. The research used a completely randomized design consisting of five treatments, namely 2 control treatments (negative control and positive control) and three treatments containing feed with Ambon banana stem extract, namely 0.5 g kg ^− 1 , 0.75 g kg ^− 1 , and 1 g kg ^− 1 of feed. Shrimp were raised for 30 days and fed five times a day. On the 31st day, shrimp were challenged with V. parahaemolyticus at a dose of 10 ⁵ CFU mL ^− 1 , except for the negative control. The results showed that the dose of 0.75 g kg ^− 1 provided significantly higher growth performance (P < 0.05) compared to other doses and controls. Then a dose of 0.75 g kg ^− 1 was able to increase immune response significantly higher (P < 0.05) than other doses and controls. The bacterial abundance in the intestine at a dose of 0.75 g kg ^− 1 also significantly suppressed the growth of V. parahaemolyticus (P < 0.05) compared to other doses and controls. In addition, the survival rate of vannamei shrimp at a dose of 0.75 g kg ^− 1 was significantly higher (P < 0.05) compared to other doses and controls. The results of this research can be used as a reference for the optimal dosage for preventing V. parahaemolyticus bacterial infections using Ambon banana stem extract.