Transcriptional responses to direct and indirect TGFβ1 stimulation in cancerous and noncancerous mammary epithelial cells.

Background : Transforming growth factor-beta (TGFβ) is important for the morphogenesis and secretory function of the mammary gland. It is one of the main activators of the epithelial–mesenchymal transition (EMT), a process important for tissue remodeling and regeneration, but also providing cells with the plasticity to form metastases during tumor progression. Noncancerous and cancer cells respond differently to TGFβ. However, knowledge of the cellular signaling cascades triggered by TGFβ in various cell types is still limited. Methods : MCF10A (noncancerous, originating from fibrotic breast tissue) and MCF7 (cancer, estrogen receptor-positive) breast epithelial cells were treated with TGFβ1 directly or through conditioned media from stimulated cells. Transcriptional changes (via RNA-seq) were assessed in untreated cells and after 1-6 days of treatment. Differentially expressed genes were detected with DESeq2 and the hallmark collection was selected for gene set enrichment analysis. Results : TGFβ1 induces EMT in both the MCF10A and MCF7 cell lines but via slightly different mechanisms (signaling through SMAD3 is more active in MCF7 cells), and many EMT-related genes are expressed in MCF10A cells at baseline. Both cell lines respond to TGFβ1 by decreasing the expression of genes involved in cell proliferation: through the repression of MYC (and its targets) in MCF10A cells and the activation of p63-dependent signaling in MCF7 cells ( CDKN1A and CDKN2B , which are responsible for the inhibition of cyclin-dependent kinases, are upregulated). In addition, estrogen receptor signaling is inhibited and caspase-dependent cell death is induced only in MCF7 cells. Moreover, direct incubation with TGFβ1 and treatment of cells with conditioned media similarly affected transcriptional profiles. However, TGFβ1-induced protein secretion is more pronounced in MCF10A cells; therefore, the signaling is propagated through conditioned media (bystander effect) more effectively in MCF10A cells than in MCF7 cells. Conclusions : Estrogen receptor-positive breast cancer patients may benefit from high levels of TGFB1 expression due to the repression of estrogen receptor signaling, inhibition of proliferation, and induction of apoptosis in cancer cells. However, some TGFβ1-stimulated cells may undergo EMT, which increases the risk of metastasis.