LncRNA H19 was a prognostic biomarker associated with immune infiltrates in stomach adenocarcinoma

Background Expression of long non-coding RNA (LncRNA) H19 was observed in many diseases, while its role in stomach adenocarcinoma (STAD) was unclear. The objective of this research was to demonstrate the relationship between H19 and STAD. Methods Kaplan-Meier (K-M) analysis was applied to analyze the variations in survival of different subgroups of H19 expression. Immunoanalysis and gene set enrichment analysis (GSEA) were utilized to filter the H19 functions that were substantially implicated. The methodology of Cox regression analysis was adopted to assess factors contributing to the prognosis. we also established an H19 ceRNA network and performed drug sensitivity analysis. In the final phase, the consistency between the expression of H19 in clinical samples and the results of bioinformatics was verified by RT-qPCR. Results High H19 expression predicted poorer survival, and plasmacytoid dendritic cells, neutrophils, and activated CD4 and CD8 T cells were correlated with the prognosis of STAD patients. The immune infiltration and GSEA analysis hypothesized that H19 expression was connected to the function of ECM receptor intraction and some differential immune cells. Independent prognostic analysis suggested that age, tumor stage, and H19 expression could act as separate prognostic factors. Ultimately, we constructed the ‘H19-3 miRNA-18 mRNA’ network. RT-qPCR results showed that the expression level of H19 in STAD tissues was significantly higher than that in adjacent normal tissues. Conclusion H19 expression was significantly connected to poor survival in STAD, and it might be a prospective biomarker for prognosis in STAD.