Lactobacillus delbrueckii ssp. indius AG1: Potential candidate for Degradation of agriculture wastes and production of biogas by orsat instrument method

The anaerobic digestion of organic materials, which is necessary to add chemicals to the continuous biochemical process, is largely mediated by microorganisms. Through a sequence of metabolic events, the microorganisms efficiently break down complex organic compounds to produce simple compounds like methane. In this investigation, inoculums used for biogas production was isolated from agriculture waste sources— L. delbrueckii ssp. Indius AG1 at mesophilic temperature (35ºC)—to assess their suitability for anaerobic digestion of agricultural residues (AR) and fruit and vegetable waste (FVW). The highest biogas production of 215 ml/2 kg waste was achieved for a mixture of inoculums, and biogas characterization by orsat instrument method showed 47.7% of the methane content. The morphological, biochemical, and molecular techniques were used to identify the microbial flora present in the high-yield reactor. The abundance of L. delbrueckii ssp. indius AG1 . To get a high methane yield from organic waste it is necessary to maintain the equilibrium and availability of efficient microbial communities like firmicutous, hydrogenotrophic, and acetoclastic methanogens.