Integrated Analysis of lncRNA–Mediated ceRNA Network in type 2 diabetes

Background: The lncRNA–miRNA–mRNA ceRNA network has been theorized to play an indispensable role in many disease,however, the function and regulatory mechanisms of ceRNAs associated with lncRNA in diabetes remains unclear.We predict the key lncRNA involved in the peripheral blood ceRNA mechanism of type 2 diabetes by correlation analysis and constructing a lncRNA-miRNA-mRNA network, to discover new diabetes markers or therapeutic targets. Methods The expression profile of differential lncRNA in peripheral blood of type 2 diabetes was detected by gene chip technology. Then use R language and bioinformatics tools to process chip data, predict the target gene by correlation analysis and construct lncRNA-miRNA-mRNA network. Then perform KEGG pathway analysis and GO enrichment analysis with lncRNA, and predict key lncRNA. Results Correlation analysis obtained 2016 pairs of relationship, including 125 lncRNA and 163 mRNA. KEGG pathway and GO enrichment analysis show that there are multiple pathways which related to the occurrence and development of type 2 diabetes. The lncRNA-miRNA-mRNA network was successfully constructed according to the results of the chip and predicted data, including 21 miRNAs, 12 mRNAs, 82 lncRNAs and 187 interaction pairs. The prediction tools screened out 6 key lncRNAs. Conclusion LncRNA may mediate the occurrence and development of diabetes by the ceRNA mechanism, and its key lncRNA may become a new diabetes screening marker or therapeutic target in the future.