Breast cancer cell-derived extracellular vesicles promote human umbilical vein endothelial cells proliferation and migration by regulating the JAK2/STAT3 pathway

Vascular endothelial cells are closely related to tumor progression, and extracellular vesicles (EVs) play an important role in this process. EVs derived from breast cancer cells also affect the biological characteristics of human umbilical vein endothelial cells (HUVECs) to promote tumor progression. However, the mechanism remains unclear. In the present study, EVs derived from MDA-MB-231 and MCF-7 breast cancer cells were extracted at different concentrations (0, 10, 20, and 40 μg/ml). Specific concentrations of breast cancer cell line EVs (10, 20, and 40 μg/ml) significantly increased the proliferation, Colony formation, and migration of HUVECs (P < 0.05) compared to MDA-MB-231 and MCF-7 breast cancer cell-derived EVs. There was no significant difference in the expression levels of total JAK2 and STAT3 protein (P < 0.05), while the expression levels of P-JAK2 and P-STAT3 protein increased significantly (P < 0.05). In conclusion, EVs derived from breast cancer cells may promote the proliferation and migration of HUVECs by regulating the phosphorylation of the JAK2/STAT3 signaling pathway.