Characterization of an efficient waste fat hydrolysing and detergent compactible lipase from newly isolated Pseudomonas mosselii

Lipase enzyme plays a major role in several industrial processes. The effective production of lipase enzyme from microorganism in a cost-effective manner is in great demand in the current scenario. This study has aimed in producing an effective and high active stable lipase enzyme from Pseudomonas mosselii isolated from the highly polluted cooum river bed soil, Chennai, Tamil Nadu, India. The enzyme showed the high specific activity of 157.94 U/mg. Further optimization studies which include, pH (6.5-7) 110.298 U/ml, temperature (35°C – 40°C) 112.388 U/ml, incubation time (36 hrs) 119.79 U/ml, effective substrate olive oil (1%) 118.05 U/ml and nitrogen source (Peptone 1.5% (w/v)), 150.74 U/ml enhanced the parameters to be considered for the high production of lipase enzyme. The purification process carried out in this study was ammonium sulphate precipitation, dialysis and column chromatography using Sephadex G-100 as a stationary phase. The characterization studies of partially purified lipase enzymes with parameters enhanced the stability study as follows: pH (6–8), temperature (30°C to 50°C), metal ions (Ca ²⁺ ) and detergent (Tween 80). The hydrolysis of the waste tallow using the produced lipase showed highest reaction ratio of 83.7% after 72 hrs at 50°C, 82.6% at 40°C and 81.2% at 30°C. The detergent compatible test confirmed that the lipase was compactible with the detergent and the stains were removed efficiently. Thus, this lipase may effectively serve as the feedstock for biodiesel production and as a detergent compactible application.