Isolation, identification and herbicidal potential of Fusarium avenaceum HY-041 from the Qing-Tibetan Plateau

In order to screen a biocontrol strain with broad-spectrum and high herbicidal activity, potential herbicidal strain HY-041 isolated from the leaves of naturally diseased Cephalanoplos setosum was tested. Based on the morphological characteristics and gene sequence analysis, the fungus was identified as Fusarium avenaceum . Herbicidal activity to 9 target weeds were tested, the results showed that the pathogenicity of strain HY-041 to different weeds in vitro was as follows: Polygonum lapathifolium > Malva verticillata > Avena fatua > Chenopodium album > Polygonum aviculare > Elsholtzia densa > Amaranthus retroflexus > Thlaspi arvense > Galium aparine , the pot experiment showed that the fermentation filtrate was highly pathogenic to C. album , E. densa and A. fatua by spray inoculation. The safety evaluation results showed that the strain was moderately susceptible to Brassica napus L., slightly infected Triticum aestivum L. and Hordeum vulgare , relatively safe to Pisum sativum L. and Vicia faba L.. The scanning electron microscope observation showed that strain HY-041 hyphae destroyed the epidermis tissue, invaded A. fatua tissue from the stoma and parasites, and propagated in the tissue to produce spores. The results showed that strain HY-041 had the potential to develop microbial biocontrol herbicide.