Development and validation of promoters expressed specifically in root tips and bases of lateral roots in rice and tobacco
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The root system plays important functions in water and nutrient absorption, significantly associated with crop yield. Development of promoters of expression vector specifically expressed in root system are indispensable in biotechnological breeding for cultivars with high yield and stress resistance. In this study, two promoters highly and specifically expressed in root tip tissues according to Rice Expression Profile Database, designated Root4 and Root6, were isolated, fused with reporter gene GUS, constructed with the vector pARS3m GUSPlus, and then the fused vector pARS3m GUSPlus-Root4 and GUSPlus-Root6 were transformed in rice and tobacco, respectively. Transgenic plants were detected by GUS staining, observation of tissue slices, ddPCR and Southern blotting. The results showed that the promoter Root4 drove GUS being highly expressed in the elongation zone of root tips and bases of lateral roots of both transgenic rice and tobacco plants, whereas Root6 could not drive GUS expression in any tissues of transgenic tobacco, but in the epidermis cell of root tips of transgenic rice. These results provide new vector types for use in plant genetic engineering and lay a foundation for enhancing the function of water and nutrient absorption and tolerance to abiotic stress in the roots of rice and tobacco.
