Combined multi-omics approaches to explore the mechanism of fecal bacteria transplantation in IgA nephropathy rats
Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Background This study investigated the therapeutic effects of fecal microbiota transplantation (FMT) on immunoglobulin A nephropathy (IgAN) using a multi-omics approach. Methods Rats were divided into control, IgAN model, and FMT intervention groups, and their kidney tissue, serum, and fecal samples were analyzed through metabolomics, microbiomics, and transcriptomics. Results Multi-omics correlation analysis identified 28 key genes, four key microorganisms, and 23 metabolites associated with kidney function. Genes like Tmed6, Pstpip1, and Pdilt, as well as metabolites such as LPS 20:3 and Etridiazole, were positively correlated with kidney function, while Pechueloic acid showed a negative correlation. Gene set enrichment analysis revealed key pathways involved in IgAN, including chemokine signaling, oxidative phosphorylation, and Toll-like receptor signaling. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) results confirmed significant expression changes in key genes between IgAN and FMT-treated groups, further supporting the therapeutic potential of FMT. Conclusions This study provides novel insights into the microbe-metabolite-gene regulatory mechanisms during FMT intervention, offering valuable directions for IgAN research and treatment strategies.
