OsEIN2-OsEIL1/2-OsNAP module regulates rice thermotolerance and leaf senescence
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High temperature and leaf senescence are critical factors affecting the growth, development, yield and quality of rice. Ethylene signaling plays an essential role in plant responses to various stresses and leaf senescence. Here, we report that the master components of ethylene signaling, OsEIN2 and OsEIL1/2, regulate rice thermotolerance and leaf senescence at the seedling stage by activating OsNAP expression. Physiological analyses showed that OsEIN2 and OsEIL1/2 triggered excessive ROS accumulation, chlorophyll degradation, and severe cell death under heat stress and dark treatment. Transcriptomic analyses revealed that many genes regulated by OsEIL1 under high temperature were involved in ROS homeostasis, leaf senescence and stress response. Our analyses revealed that OsEIL1/2 could directly transcriptionally activate OsNAP - mediated rice thermosensitivity and leaf senescence. Moreover, we found that OsEIL1/2 repressed the expression of OsEIL5 , a positive regulator of thermotolerance, implying that different EIN3-like homologs might have antagonistic effects on rice response to heat stress. Taken together, our results provide new insights into the OsEIN2-OsEIL1/2- OsNAP module involved in regulating rice thermotolerance and leaf senescence, and offer genetic resources for developing heat-tolerant rice cultivars.
