Proteomic Analysis of Two Stem Cell Secretomes Used in Combination in a Topical Skin Care Product That Has Demonstrated Efficacy in a Double-Blinded, Vehicle-Controlled Clinical Trial

We performed a proteomic analysis of the secretome of two stem cell types currently used in topical skin care product that has proven benefits as evidenced by a double-blinded, randomized, vehicle- controlled clinical trial. Our analysis was of the whole secretome, not just constituent parts such as exosomes and/or ectosomes, given the complete secretome compared to only its parts has been found to be more beneficial to reducing inflammation and eliciting a regenerative state in tissues. While many protein types were common to the two cell types, adipose mesenchymal stem cells, fibroblasts, significant differences were found between the two, providing evidence for the mechanisms of actions of each. The proteome we analyzed in the combination secretome of the two cell types provides at least 16 major therapeutic pathways comprised of 100s of signaling mechanisms to provide many benefits to various skin conditions when topically applied.