Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study II

Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were tested by using a cell culture model. The several components of dentin adhesives like primer and bonding were analyzed as single and additive applied components as specified by the manufacturer for the application in-vivo. 75 petri dishes were produced per group and all petri dishes (480 ones) were evaluated triangulated. This unique assessment is following our first investigation and the observation period is extended from 24 hours to 48 hours. AdheSE, Clearfil SE Bond, One-up Bond F Plus and Optibond Solo Plus showed statistically significant less amounts of viable cells compared to the cell control. All dentin adhesives except Clearfil SE Bond showed a statistically significant difference regarding the reactivity index in the application comparison. In conclusion, the test materials showed a moderate grade of cytotoxicity with no statistically significant difference regarding the cytotoxicity between the tested self-etch and etch-and-rinse dentin adhesives. However, the results show differences between sequentially and single applied adhesive parts.