Microenvironment Rheology Modulates the Effect of the Anticancer-Peptide CIGB300 on 3D Head and Neck Tumoroids
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3D cell systems for in vitro experimental studies are able to mimic the in vivo efficacy of drugs, before testing on animals, however many studies are still needed for mimicking the physiological environment with 3D cell growth systems. The mechano-physical properties of the microenvironment are relevant for the invasiveness of cancer cells and also for their drug resistance. 3D tumoroids of human oral squamous cell carcinoma (OSCC) CAL27 cells of different stiffness are here produced using a tunable PEG-silk fibroin hydrogel (PSF) and the anti-tumor activity of the CIGB300 peptide, an anticancer therapeutic peptide, on these 3D tumoroids models is assessed. Moreover, spectroscopical studies on CIGB300 peptide are here also reported for analyzing its structure, stability, aggregation and diffusion properties. For the first time, the CIGB300 peptide diffusion in tunable silk fibroin hydrogels of different stiffness is investigated over-time by fluorescence spectroscopy as potential tool in drug-screening using hydrogel-based 3D tumoroids.