Hypothetical Hybrid Integration of Ozonated Water Disinfection with Biotechnological <em>Aedes aegypti </em>Control for Dengue Suppression in Jeddah: Simulation-Based Extension and Analysis

Dengue fever remains a pressing public health issue in Jeddah, Saudi Arabia, with increasing incidence driven by urban Aedes aegypti proliferation. Building on our prior review and protocol for biotechnological suppression using Wolbachia and genetic methods, this hypothetical extension explores integrating ozonated water disinfection as a complementary environmental intervention. Using an updated simulation-based Design-of-Experiments (DoE), we model hybrid strategies incorporating ozone-induced larval mortality (ϕo = 0.1–0.5 day−1) into a compartmental SEIR-SEI framework. Hypothetical outcomes from 20 Latin Hypercube-sampled scenarios show average 91% dengue incidence reduction, with high-ozone scenarios achieving near-elimination (98–99%) and rapid Wolbachia replacement (100% by day 40). Time-series analyses reveal initial vector suppression followed by stabilization, highlighting synergies for eco-friendly control. This simulation informs potential field pilots, addressing ecological concerns while enhancing efficacy in Jeddah’s context. Limitations include hypothetical assumptions; empirical validation is recommended.