Protocol for Quantifying Silent Ribosome Induction Using Polysome Profiling
Discuss this preprint
Start a discussion What are Sciety discussions?Listed in
This article is not in any list yet, why not save it to one of your lists.Abstract
Translationally silent ribosomes have become an important focus in RNA biology. Lacking mRNAs, they represent a hibernating ribosome state. Their quantification remains challenging. This protocol outlines how to measure silent ribosome induction under diverse conditions by analyzing RNA from polysome-profiling fractions in yeast and mammalian cells, using RNA-seq with detection and removal of anomalously amplified rRNAs, complemented by qPCR for validation. For an example of the execution of this protocol, please refer to Rahaman, et. al [1].