Antigout and Antidiabetes Potentials of <em>Celastrus hindsii</em>

Background/Objectives: Given the growing global health threat posed by gout and diabetes, studying medicinal plants like Celastrus hindsii for their potential in supporting and treating these diseases is highly valuable. Methods: In this study, Celastrus hindsii leaves were extracted using different solvents, and their antihyperuricemic, α-amylase, and α-glucosidase inhibitory activities were evaluated. To gain deeper insights into the medicinal potential of this plant, gas chromatography - mass spectrometry(GC-MS) and Electrospray Ionization Mass Spectrometry (ESI-MS) analyses were performed to identify the bioactive compounds present in the extracts. Results: The results indicated that the ethyl acetate (EtOAc) extract demonstrated the most potent inhibitory effects against XO (IC₅₀ = 114.06 µg/mL), α-amylase (IC₅₀ = 363.46 µg/mL), and α-glucosidase (IC₅₀ = 689.09 µg/mL). Furthermore, the EtOAc extract was fractionated using column chromatography for detailed analysis. Among the fractions obtained, fraction V5, eluted with CHCl₃:MeOH (99:1), exhibited the strongest inhibitory activity against XO (IC₅₀ = 38.22 µg/mL), α-amylase (IC₅₀ = 68.00 µg/mL), and α-glucosidase (IC₅₀ = 293.22 µg/mL). Gas chromatography–mass spectrometry (GC–MS) and electrospray ionization mass spectrometry (ESI–MS) analyses revealed a substantial amount of fucosterol in the leaves of C. hindsii (2.03 mg/g extract), with the highest concentration detected in fraction V5 (1.08 mg/g fraction). Conclusions: This research has opened up the promising avenue for the development of C. hindsii as a valuable source for medicinal and functional food products.