Therapeutic Potential of Flavonoids and Rutin Against Inflammation on a Huntington Animal Model

Purpose. This study tested the hypothesis that Flavonoids (diosmine, hesperidin and rutin®) and induced inflammation lead to the protection of brain and duodenum against free radicals as consequence of 3-NPA administration by measuring the levels of GABA, 5-HIAA, dopamine and some inflammation and oxidative stress markers. Study design. Male young Wistar rats (weight 60g) received Salmonella thyphimurium ATCC14028 1x106 UFC/g every week, for two consecutive weeks, plus the following treatments: group A, NaCl 0.9% (Control); group B, mix flavonoids + rutin® (300mg of mix diosmine, 33.3mg of hesperidin and 150mg/kg weight of rutin; group C, 3-NPA; group D, 3-NPA + mix flavonoids + 1ml of rutin® per rat. 3-NPA administration was at 24mg/kgW by intraperitoneal route, while flavonoids at every 48 hours for 15 days by oral route. Methods. At the moment of sacrifice, the blood was obtained to assess Interleukine-6, glucose, triglycerides and haemoglobin levels. Brain and duodenum were obtained to measure GABA, dopamine, 5-HIAA, lipoperoxidation, reduced glutathione (GSH), total ATPase concentrations and catalase activity using validated methods. The brains, stomach and duodenum were dissected for histological analysis. Results. In group A, there is a decrease in interleukine-6 levels (p=0.009). In the cortex region of animals in group C, dopamine experienced a significant decrease (p=0.012). GABA increased (p=0.001) in cerebellum regions of animals in the groups A, B and C. ATPase activity increased (p = 0.013) in cerebellum regions in animals of groups C and D. Lipoperoxidation diminished (p=0.043) in cerebellum region of group D. Catalase activity diminished (p=0.043) in Cortex region of animals in groups B and D groups, and (p=0.002) in cerebellum region of animals in groups A and B. Besides, histological changes revealed marked lesions of neuronal cells in experimental animals treated with nitro propionic acid. Conclusion. The protective role of flavonoids and rutin compounds on inhibition of the inflammatory response and correction of the fundamental oxidant/antioxidant imbalance in animals suffering from Huntington diseases are important vistas for further research.