A Bio-Fortified Whole Tomato Food Supplement as Potential Dietary Tool for the Management of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)

Background: Western diets, rich in refined fats and carbohydrates, are recognized as a major player in hepatic lipid accumulation in adults and youngsters, leading to the growing prevalence of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD), the gate to cirrhosis and cancer. Due to the lack of efficacious therapies, antioxidant-rich dietary regimens targeting different pathologic pathways may be clinically advantageous. Objective: As tomatoes are a major available source of antioxidant/inflammatory nutrients, we have investigated whether a novel whole tomato-based food supplement (WTFS), possessing an effective antioxidant activity and hindering multiple metabolic pathways, can interfere with mechanisms fostering MASLD progression. Methods: Lipidomic and proteomic analyses were performed in the HepG2 liver human cell line treated with WTSF. Results: WTFS induces a marked reduction in triglycerides and cholesterol ester content, a decrease in the relative levels of diacylglycerols, lysophosphatidylcholine, lysophosphatidylethanolamines, phosphatidylethanolamines, and lower expression of transforming growth factor-α, tumor necrosis factor-like weak inducer of apoptosis (TWEAK), and Fms-related tyrosine kinase 3 ligand (FLT3LG), signaling relevant to MASLD progression. Conclusions: WTFS may represent a potential candidate for clinical trials in supplementing antioxidant-rich dietary regimens such as the healthy but hard-to-follow Mediterranean diet, the presently first-line preventive and therapeutic nutritional regimen for MASLD.