Salivary Biomarker Analysis to Distinguish between Health and Periodontitis Status: A Preliminary Study

Objective: This study aims to explore the feasibility of a non-invasive and simple method to discriminate between health and periodontitis (PRD), facilitating early and objective diagnosis of PRD before the detectable periodontal attachment loss and monitoring treatment outcomes. Design: Salivary samples were collected from 16 PRD-free patients (G1) and 10 pa-tients with PRD (G2). The analysis included salivary matrix metalloproteinase-8 (MMP-8), major anti-inflammatory interleukins (IL-4 and IL-10), pro-inflammatory cytokines (IL-1β, IL-8, and interferon α [IFN-α]), and the cytokine IL-6. Clinical and salivary assessments were performed at baseline (TP0) for both groups, and after periodontal treatment for G2 (TP1). Results: PRD indices were significantly higher in G2-TP0, lower in G1, and interme-diate in G2-TP1. Except for IL-6, the biomarkers were significantly correlated with nearly all PRD clinical indices. Logistic regression and ROC curve analyses showed statistical significance for MMP-8, IL-1β, IL-4, IL-8, and IL-10 when comparing G1 and G2 at TP0. MMP-8 was also significant when comparing G2-TP0 and G2-TP1, while IL-1β and IL-10 showed borderline significance. IL-8 was significant when comparing G1 and G2-TP1. Conclusion: The molecular network demonstrated great potential for early diagnosis and monitoring of therapy response, providing a promising basis for future research. Among the biomarkers, MMP-8, IL-1β, IL-4, IL-8, and IL-10 showed the strongest statistical correlations with the clinical indices. The inflammation-related biomolecules behaved differently among untreated PRD (G2-TP0), treated (G2-TP1), and healthy individuals (G1). Healthy individuals and those with treated PRD may regulate inflammation significantly differently from those with untreated PRD.