Heparan Sulfate Proteoglycans Mediate In Vitro Human Neuronal Lineage Specification

Heparan sulfate proteoglycans (HSPGs) within the neuronal niche are expressed during brain development, con-tributing to multiple aspects of neurogenesis, yet their roles in glial lineage commitment remain elusive. This study utilised three human cell models expanded under basal culture conditions followed by media induced lineage in-duction to identify a reproducible and robust model of gliogenesis. SH-SY5Y human neuroblastoma cells (neuronal control), ReNcell CX human neural progenitor cells (astrocyte inductive) and ReNcell VM human neural progenitor (mixed neural induction) models were examined. Cultures were characterised during basal and inductive states via Q-PCR, Western Blotting, immunocytochemistry (ICC), and calcium signalling activity analyses. While the ReNcell lines did not produce fully mature or homogeneous astrocyte cultures, ReNcell CX cultures most closely resembled an astrocytic phenotype with ReNcell VM cells treated with platelet derived growth factor (PDGF) biased toward an oligodendrocyte lineage. The glycated variant of surface-bound glypican-2 (GPC2) was found to be associated with lineage commitment, with GPC6 and 6-O HS sulfation upregulated in astrocyte lineage cultures. Syndecan-3 (SDC3) emerged as a lineage-sensitive proteoglycan, with its cytoplasmic domain enriched in progenitor-like states and lost upon differentiation, supporting a role in maintaining neural plasticity. Conversely, the persistence of transmem-brane-bound SDC3 in astrocyte cultures suggest continued involvement in extracellular signalling and glycan secretion, demonstrated by increased membrane-bound HS aggregates. This data supports HSPGs and HS GAGs as human neural lineage differentiation and specification markers that may enable better isolation of human neural lineage specific cell populations and improve our understanding of human neurogenesis.