Title: Enhancing Soil Health and Corn Productivity with a Co-Fermented Microbial Inoculant (CFMI-8): A Field-Based Evaluation

Soil degradation and declining fertility threaten sustainable agriculture and crop productivity. This study evaluates the effects of CFMI-8, a co-fermented microbial inoc-ulant comprising eight bacterial strains selected through genomic and metabolic mod-eling, on soil health, nutrient availability, and corn performance. Conducted in a ran-domized complete block design at Findlay Farm, Wisconsin, the field trial assessed soil biological activity, nutrient cycling, and crop yield responses to CFMI-8 treatment. Treated soils exhibited significant increases in microbial organic carbon (+224.1%) and CO₂ respiration (+167.1%), indicating enhanced microbial activity and organic matter decomposition. Improvements in nitrate nitrogen (+20.2%), cation exchange capacity (+23.1%), and potassium (+27.3%) were also observed. Corn yield increased by 28.6%, with corresponding gains in silage yield (+9.6%) and nutritional quality. Leaf micronu-trient concentrations, particularly iron, manganese, boron, and zinc, were significantly higher in treated plants. Correlation and Random Forest analyses identified microbial activity and nitrogen availability as key predictors of yield and nutrient uptake. These results demonstrate CFMI-8’s potential to enhance soil fertility, promote nutrient cycling, and improve crop productivity under field conditions. The findings support microbial inoculants as viable tools for regenerative agriculture and emphasize the need for long-term studies to assess sustainability impacts.