Effects of Cavity Depth (Moderate vs Deep with Pulp Exposure) on the Release of Prostaglandin E2 and Nitric Oxide in Rat Mandibular Incisors

Background/Objectives: Inflammatory mediators such as prostaglandin E2 (PGE2) and nitric oxide (NO) are key indicators of pulp response to mechanical trauma. However, the influence of cavity depth on their release dynamics remains unclear. This study aimed to evaluate the effects of different cavity depths—moderate (without pulp exposure) and deep (with pulp exposure)—on the release of PGE2 and NO in the pulp tissue of rat mandibular incisors at two time intervals (3 and 9hr). Methods: Forty male Wistar rats were divided into two main groups (n=20) based on cavity depth. A split-mouth design was used, with cavities of different depths prepared on the mandibular left incisors while leaving the right incisors without cavities as controls. All prepared cavities were sealed with glass ionomer filling until 3 or 9 hours (n=10), when pulp tissue was removed. Homogenates were prepared and analyzed by ELISA for PGE2 and NO levels. Results: Both mediators showed significantly higher concentrations in cavity groups than controls. Notably, cavities without pulp exposure elicited greater PGE2 and NO release than those with exposure. Time interval (3 vs. 9 hours) did not significantly affect the mediator levels. Conclusion: Cavity preparation induces an early inflammatory response in the pulp, as indicated by increased PGE2 and NO levels. The inflammatory response was more pronounced in cavities without pulp exposure, suggesting that maintaining pulp integrity may favor a more immune reaction conducive to healing.