Substrate Formulation Effects on Pleurotus ostreatus Production and Nutritional Profile

This study compared the effects of using different lignocellulosic substrates on the growth, yield, and nutritional composition of Pleurotus ostreatus mushrooms. Three substrate formulas were investigated: a commercial/standard oyster formula (S), willow chips with cottonseed hulls (W+C), and willow chips alone (W). Cultivation trials, in-cluding incubation and cropping stages, were conducted at a suboptimal temperature of 15°C to assess the feasibility of mushroom production under cool climate conditions, such as those anticipated during a nuclear winter, where there is limited energy available for food production. Results indicated that substrate composition significantly influenced yield, biological efficiency, and digestible protein content of P. ostreatus mushrooms. However, no noticeable differences were observed in total colonization period or time to pinning and harvest among treatments. Substrate S and W+C were the most suitable for oyster mushroom cultivation in terms of fresh mushroom yield and biological efficiency. Crude protein, B vitamins, and mineral content did not differ significantly across treatments. This study highlights the potential of P. ostreatus as a sustainable food source in low-temperature environments with minimal energy input. Its ability to thrive on woody substrate and its low cultivation energy requirements make it a viable, nutrient-rich option for enhancing food security in post-catastrophic scenarios.