Enhanced Isolation of Brucella abortus from Lymphoid Tissues of Mice Orally Infected with Low Doses in a Two-Step Procedure
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The main aspects of brucellosis have been studied in animal models to better understand the pathogenesis of the disease and develop vaccines and drugs for its treatment. Mice are the most common animal model of brucellosis. To verify that the infection has been successfully induced, it is necessary to assess the presence of Brucella spp. in the experimentally infected mice. The most common way to detect the presence of Brucella is by microbiological isolation from spleen and liver. However, high doses of Brucella are needed by most models to successfully isolate the bacteria. In this study we propose a method consisting in culturing homogenates of the organs recovered from orally infected mice in selective broth to increase the bacterial load. This made it possible to recover Brucella abortus from Peyer’s patches, mesenteric lymph nodes, spleen, and feces of mice orally inoculated with a low dose of 5 x 106 CFU, as early as one hour and up to five weeks post inoculation.