Micropropagation of ‘Manacá-De-Cheiro’ (<em>Brunfelsia uniflora </em>(Pohl) D. Don), an Ornamental Species Native to Brazil

The introduction of new ornamental species and cultivars has been one of the hallmarks of innovation in global floriculture. Brunfelsia uniflora is a subshrub species native to Brazil, with white, lilac, and blue flowers on the same plant, in addition to a characteristic perfume. As it is still a wild species, technologies such as large-scale clonal propagation of superior genotypes are still scarce to supply the flower market. In this way, a successful protocol for the micropropagation of B. uniflora was developed using nodal segments and shoot tips as initial explants. In the multiplication phase, the use of 6-Benzylaminopurine resulted in the highest multiplication rates (10.3-10.9 shoots/explant) and the number of leaves in the shoots. The in vitro shoot rooting was superior using MS medium containing reductions of the macronutrients and added with IBA, totaling until 91.7% rooted shoots. The greatest difficulty in micropropagation of this species was related to the high percentage of shoots containing calli; the highest percentage of calli was found with the addition of auxins at the high concentrations (1.0 and 1.5 mg L-1). The even so, shoots and plantlets were acclimatized, demonstrating the success of this technique for the production of plantlets of B. uniflora.