Tackling Critical Issues in scRNA-seq Data Normalization and Bulk RNA-seq Data Cell Type Deconvolution

We recently developed a novel method, ReDeconv, for the normalization of scRNA-seq data and cell type deconvolution within bulk RNA-seq data. Our manuscript on ReDeconv, titled "Transcriptome Size Matters for Single-Cell RNA-seq Normalization and Bulk Deconvolution", was published in Nature Communication in February 2025. Through this work, we explored various factors that significantly influence scRNA-seq data normalization and bulk RNA-seq data deconvolution, and we proposed innovative solutions to tackle these issues. Recognizing the critical nature of these issues, we present a summary of our work in a Question & Answer (Q&A) format to underscore their importance.