Synergistic Reduction of Staphylococcus aureus Biofilms Using Povidone Iodine and Hydrogen Peroxide

Staphylococcus aureus, particularly its methicillin-resistant strains (MRSA), poses significant challenges in healthcare settings due to its propensity to form biofilms on various surfaces. These biofilms enhance bacterial survival and increase resistance to conventional treatments, complicating infection control efforts. This study investigates the efficacy of a novel approach combining povidone-iodine (PVP-I) and hydrogen peroxide (H₂O₂) to combat Staphylococcus aureus biofilm formation in hospital environments. A range of methodologies were employed, including biofilm formation assays, gene expression analysis, colony-forming unit (CFU) enumeration, and confocal microscopy, to assess the effects of PVP-I and H₂O₂, both individually and in combination. The results demonstrated a synergistic effect when these agents were combined, significantly reducing biofilm formation compared to the use of either compound alone. PVP-I, an io-dine-based antiseptic, and H₂O₂, a potent oxidizing agent, each exhibit potential in disrupting bacterial biofilms when used independently. However, their combined application appears to enhance penetration into the biofilm matrix and increase overall antimicrobial activity. These findings have important implications for infection control strategies in healthcare settings. By harnessing the complementary mechanisms of PVP-I and H₂O₂, this approach offers a promising solution for preventing and eradicating biofilm-associated infections on medical devices and hospital surfaces. Additionally, the use of two agents with different modes of action may help mitigate the risk of developing antimicrobial resistance. This study lays the groundwork for future research aimed at optimizing concentrations, application methods, and assessing the long-term efficacy and safety of this combined approach. Ultimately, this research contributes to improved patient outcomes and a reduction in the economic burden associated with biofilm-related complications.