Purification and Characterization of the Recombinant Chitinase ChiBlUV02 of Bacillus licheniformis UV01 with Choleoptericidal Effect on the Hive Beetle (Aethina tumida)
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The biotechnological applications of chitinases are diverse, they are used in industrial sectors such as pharmaceutical and textile, as well as in agriculture, including the use of recombinant chitinases for pest control, since traditional treatments affect and contaminate hive products. Bacillus licheniformis UV01 is an bacterium of interest that expresses genes for different enzymes, including chitinase. The Chibluv01 gene was cloned into the pHTP8 vector with a His/tag, for purification by affinity chromatography. Showing a specific activity of 115 U/mL, the optimal pH of the enzyme was pH 7.5, and an optimal temperature of 42 ºC. The choleoptericidal activity of the enzyme was evaluated in larvae and adult beetles of Aethina tumida treated by immersion in a purified enzyme extract at different concentrations, verifying mortality at 24, 48 and 72 hours. Within 24 hours of application, there was a higher mortality of 50 % in the larval stage and 56.6 % in adult beetles compared to the control groups. LC50 and LC90 were obtained: 104.05 U/mL and 234.36 U/mL in larvae and 92.99 U/mL and 211.14 U/mL in adults. This results indicate the potential of the application od ChiBlUV02 chitinase in pest control.