Ecofriendly Extraction of Potential Cosmetic Ingredients from Colombian Marine Macroalgae, Using Natural Deep Eutectic Solvents (Nades)

This work studied the extraction of bioactive metabolites from twelve Colombian marine macroalgae samples. Macroalgae samples were collected from the northeast coastal areas of Colombia and extracted using ultrasound-assisted liquid extraction with different Nades and compared them with reference solvents. The analysis of the extracts in-cludes preliminary chemical tests such as Folin-Ciocalteu quantifica-tion, DPPH assays, and MAAs identification using HPLC-DAD. In ad-dition, exploratory physicochemical tests measured the porosity of the sprayed material using the B.E.T. method and evaluated the Nades flow profile. HCl 5% extracts showed the highest yield of total phenolic compounds. It was 3-4% w/w for Gracilariopsis lemaneiformis, Hypnea sp., Pterocladiella capillacea, and Stypopodium zonale. Nades Beta-ine/Glucose/Water 1:1:5 had the highest efficiency extracting total phenolic compounds in most species. Over Sargassum polyceratium increasing the extraction scale, improved the recovery rate from 0 to 24 hours. The experiment reached saturation with 10 mL of BGluW115. It reproduced this result with different batches of glucose and betaine. MAAs concentrations ranged from 0.2 to 0.5 mg·g⁻¹ DW in the species analyzed with aqueous extractions. Nades FGW115 and BGW 115 allowed the detection of MAAs. Porosity differences among macroalgae did not affect phenolic recovery. Finally, Nades flow profiles exhibited Newtonian behavior.