Efficacy of Lincomycin HCl Loaded Chitosan Nanoparticles Gel for In-Vivo Wound Therapy in Female Rats Model: Revolutionizing Tissue-Regeneration and Cutaneous Applications

This study revolves around the design/optimization of nanoparticles containing Lincomycin HCl, utilizing chitosan as a polymer and sodium tripolyphosphate as a cross-linker. The ionotropic gelation method was employed for nanoparticle preparation. An optimized formulation was subjected to accelerated stability studies and evaluated through various parameters including particle size (103 ± 43 nm), zeta potential, scanning electron microscopy, and Fourier transform infrared spectroscopy, differential scanning calorimetry, Scanning Electron microscopy (SEM/TEM) and powdered X-ray diffraction. The entrapment efficiency of nanoparticles increased with rising drug concentration up to 0.2 g. FTIR and thermal studies analysis confirmed the absence of interactions between the drug and other components. X-ray diffraction analysis indicated the amorphous nature of Lincomycin HCl within the nanoparticles. Accelerated stability assessment demonstrated the integrity of the formulation. Moreover, Lincomycin HCl effectively prevented rat infections compared to control groups during a two-week study. The LD50 of Lincomycin HCl in rats surpassed 100 mg/kg, with acute toxicity analysis revealing no significant changes between untreated and Lincomycin HCl-treated rats. Histopathological examination indicated no damage to heart, liver, or kidney tissues. Thus, it is reasonable to assert that Lincomycin HCl demonstrated substantial antimicrobial activity in rats, supporting its traditional medicinal usage.