The Effects of Artificial Diets Containing Free Amino Acids Versus Intact Proteins on Biomarkers of Nutrition and Deformed Wing Virus Levels in the Honey Bee

Pollen is bees' primary source of proteins. Using pollen substitutes could reduce colony losses in areas with limited floral resources. In this study, we compared the effects of pollen substitutes made from intact proteins versus free amino acids on bee survival, as well as the levels of vitellogenin (vg), major royal jelly protein 1 (mrjp1), and deformed wing virus (DWV). Our study revealed no significant differences in vg mRNA levels between bees-fed intact protein and free amino acid diets. However, mrjp1 mRNA levels were higher in bees-fed free amino acid diets, suggesting that the availability of amino acids affects the expression of this nutritionally regulated gene differently. Thus, the combined expression of vg and mrjp1 could be used to assess the nutritional value of different pollen substitutes. Our results also indicate that bees fed diets rich in free amino acids exhibited high levels of DWV and increased mortality during the first week, even though they showed high expression of genes related to good nutrition. In contrast, bees fed an intact protein diet showed the lowest DWV levels during the first two weeks, although viral infection increased afterward. These results suggest that ingested free amino acids are quickly absorbed in the intestine, transported in the hemolymph, and taken up by cells, where they can facilitate viral replication. On the other hand, ingested intact proteins may undergo slower digestion and gradual release of amino acids in the hemolymph, which may not trigger an overt DWV infection. Possible interactions among Varroa mites, DWV infection, and nutrition are also discussed.