5,6-Dihydro-5,6-Epoxymultiplolide A, Cytosporone C and Uridine Production by Diaporthe hongkongensis an Endophytic Fungus from Minquartia guianensis

Endophytic fungi are valuable sources of bioactive secondary metabolites, with potential applications in pharmaceutical and agricultural fields. This study explores the metabolic potential of Diaporthe hongkongensis, an endophytic fungus isolated from Minquartia guianensis. The fungus was cultivated on parboiled rice under static and dark conditions for 28 days, leading to the isolation of three compounds: 5,6-dihydro-5,6-epoxymultiplolide A (1), cytosporone C (2), and uridine (3). Structural identification was carried out using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The results revealed the metabolic versatility of D. hongkongensis, as demonstrated by its ability to produce structurally diverse secondary metabolites with biological relevance. The identification of a known antifungal compound and a lactone derivative underscores the biosynthetic potential of this endophytic fungus, while the isolation of a nucleoside expands the chemical repertoire of fungal metabolites, suggesting possible roles in cellular metabolism and stress adaptation. These findings reinforce the role of endophytic fungi as prolific sources of structurally diverse and potentially bioactive natural products, supporting further exploration of their biotechnological applications.