Comparative Bioavailability Study of Jaspine B: Impact of Nano-Liposomal Drug Delivery System on Pharmacokinetics

Jaspine B, an anhyrophytosphingosine, has potent anticancer properties. However, it exhibits low oral bioavailability, reducing therapeutic effects despite its cytotoxicity. This study aimed to address this issue by improving the pharmacokinetics of Jaspine B through liposomal formulation. Jaspine B liposomes were prepared using microfluidic techniques and characterized using transmission electron microscopy (TEM). A sensitive and selective LC-MS/MS method was developed and validated to quantify Jaspine B concentrations in the rat plasma. The analytical method showed linearity in a wide range of concentrations with high precision. Sprague Dawley rats were used to carry out a pharmacokinetic study to assess the impact of liposomal formulation on the pharmacokinetic parameters of Jaspine B. The liposomal formulation successfully improved the PK of the drug with enhanced bioavailability, increased half-life, and circulation time in the plasma, leading to improved therapeutic outcomes.