Polyphenols Profile, and In Vitro Antioxidant and Anticholinesterase Activities of Romanian Wild-Grown <i>Acer</i> spp. (<i>Sapindaceae</i>)
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Our paper investigates for the first time, the polyphenols profile and in vitro antioxidant and anticholinesterase activities of the leaves and fruits of Romanian wild-grown Acer spp. (Sapindaceae): A. campestre L., A. monspessulanum L., A. platanoides L., A. pseudoplatanus L., A. tataricum L. High-performance liquid chromatography/ultraviolet/mass spectrometry (HPLC/UV/MS) technique was used for the identification and quantification of phenolic acids. The spectrophotometric method was applied for the assessment of total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) antioxidant assays. In situ antioxidant properties were determined on high-performance thin-layer chromatography (HPTLC) plates after DPPH derivatization. Acetylcholinesterase inhibition effect was evaluated using microplate-based assay. Considering the polyphenols profile of Acer spp., our research highlights new natural resources with in vitro antioxidant and anticholinesterase activities.