Hepatoprotective Activity of Nature-Derived Polyphenols Studied by Mass Spectrometry Based Multi-OMICS Approach

The aim of this study was to examine the hepatoprotective activity of multicomponent mixtures of natural origin in the BALB/C mice model with subacute liver failure (SALF) induced by the administration of toxin carbon tetrachloride (CCl4). The hepatoprotective activity of activated hydrolytic lignin (BP-Cx-1), humic acid peloids (HA) and isoflavones from kudzu, Pueraria lobata (IFL) roots was evaluated using mass spectrometry based omics technologies. MASS SPECTROMETRY BASED multi-OMICS analysis revealed new insight on the molecular mechanism of the hepatoprotective activity of multicomponent mixtures of natural origin. The significant differences were observed in proteome and metabolome profiles of urine and liver of BALB/c mice with SALF between control group with CCl4 administration, intact control, and groups receiving potential hepatoprotectors of natural origin (BP-Cx-1, HA, IFL). Proteomic and metabolomics analysis demonstrated that among samples IFL possessed the highest hepatoprotective potential, which correlated well with the relative effectiveness of the drugs recorded during in vitro studies. The results correlate with the relative effectiveness of the drugs recorded in previous in vitro and in vivo studies. Leading IFL activity may be attributed to a higher content of active polyphenolic components compared to heterogeneous HA and BP-Cx-1. Enrichment with active components by fractionation is a direction, which can be explored for developing hetepoprotective agent based on natural complex polyphenols